. 2019 Jun 16;294(31):11934–11943. doi: 10.1074/jbc.RA119.008889

Table 3.

Sequences of primers used to PCR-amplify ltp2, chsH2_DUF35, and to create mutations in ltp2

NdeI and HindIII restrictions sites in primer sequences are underlined. Replaced codons in mutagenic primers are shown in boldface.

	Sequences
ltp2	`GCCCCATATGAGCGTGCTGCCCGGAGCC`
ltp2	`CGCCAAGCTTTCGGTCCGCTCCCAGGATCA`
chsH2_DUF35	`CCGGCATATGCGCCCGGCGATCAACCGCGAC`
chsH2_DUF35	`CGCCAAGCTTCATCGGTCCGCTCCCAGGATC`
H296A	`CTGTACGACGCCTTCACCCCCTGGTGCTGCCGCAACTGG`
H296A	`GGGGTGAAGGCGTCGTACAGGATGGCCGCGCCGATGTCG`
H346A	`CCTACATCGCAGGCATGAACGGCATCG CCGAGG`
H346A	`TCATGCCTGCGATGTAGGCCTCGCCGAGCTGGC`
Y294F	`CCATCCTGTTCGACCACTT CACCCCGCTGGTGCTG`
Y294F	`AAGTGGTCGAACAGGATGG CCGCGCCGATGTCGGA`
Y344F	`GCGAGGCCTTCATCCACGGCATGAACGGCATCGCCGAGGC`
Y344F	`CGTGGATGAAGGCCTCGCCGAGCTGGCCGCCGTGG`