Figure 6.

In vitro DNA ejection of myovirus Det7 and podovirus P22 after pre-incubation with LPS receptor at nonpermissive temperature. Fluorescence was monitored in the presence of S. Typhimurium LPS at 25 °C. Mixtures were heated to 37 °C after different waiting times. A, 8 × 10⁹ Det7 pfu mixed with 67 μg ml⁻¹ LPS and heated after 2 min (green circles), 17 min (white squares), and 30 min plus the addition of new LPS receptor (67 μg ml⁻¹) (cyan triangles). B, 7.2 × 10⁹ P22 pfu mixed with 10 μg ml⁻¹ LPS at 25 °C (white triangles) or with heating after 17 min (black circles). For comparison, ejection at 37 °C is shown for both phages (white circles).