Figure 4.

MaMmp10 activity is stimulated by cobalamin. Reaction products of as-purified MaMmp10 are in the presence of MeCbl or OHCbl. SAH (orange bars), 5′-dAH (purple bars), and methylated peptide product (green bars). Reactions were performed in triplicate at 30 °C in a final volume of 200 μl, and they contained the following components: 50 mm HEPES, pH 7.5, 200 mm KCl, 40 μm MaMmp10, 1 mm Ti(III) citrate, and 55 μm peptide substrate. Reactions were initiated with 1 mm SAM and quenched after 30 min. Error bars reflect standard deviation.