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. 2019 Mar 8;5(3):345–360. doi: 10.1002/vms3.158

Table 3.

Effects of egg yolk (EY) 20% and soybean lecithin (SL) at different concentrations (1%, 3% and 5%) in Tris‐citric‐fructose (T) or Tris‐citric‐fructose‐mineral salts (T‐M) extender on total motility (TM) and progressive motility (PM) parameters of chilled canine sperm during a storage period of 10 days at 5°C

Parameters Extenders Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Day 7 Day 8 Day 9 Day 10
TM (%) T‐EY 89.4 ± 1.9aA 85.4 ± 1.7aAB 83.4 ± 2.7aAB 81.1 ± 2.6aB 80.1 ± 3.4aB 77.7 ± 4.5aB 76.7 ± 4.9aB 74.9 ± 4.7aBC 69.5 ± 5.6aCD 65.2 ± 5.1aD
T‐SL1% 90.6 ± 2.5aA 87.5 ± 0.8aAB 84.9 ± 2.8aAB 79.5 ± 3.3aB 73.8 ± 1.0bC 71.6 ± 1.3aC 55.9 ± 4.5bcD 15.0 ± 4.4dE 12.1 ± 3.1cE 2.1 ± 0.9cdF
T‐SL3% 92.2 ± 0.8aA 89.3 ± 1.8aAB 85.2 ± 2.2aAB 82.8 ± 1.4aB 80.2 ± 2.3aB 74.1 ± 1.8aC 54.8 ± 6.7cD 26.1 ± 5.5cE 15.2 ± 1.9cF 5.2 ± 2.0cG
T‐SL5% 88.8 ± 3.5aA 85.0 ± 5.2aA 77.6 ± 4.7aB 72.2 ± 4.3bB 53.2 ± 4.1cC 46.8 ± 4.9bD 19.2 ± 3.1dE 5.9 ± 1.3deF 2.1 ± 0.9dF 0.0 ± 0.0dF
T‐M‐EY 85.9 ± 3.8abA 83.7 ± 2.5aAB 81.6 ± 3.1aAB 79.1 ± 2.7aB 75.7 ± 1.8abBC 71.8 ± 3.4aCD 65.2 ± 5.1bD 37.5 ± 8.0bE 25.1 ± 5.4bF 13.0 ± 2.3bG
T‐M‐SL1% 85.7 ± 3.2abA 58.9 ± 8.7bB 48.2 ± 6.5bC 12.3 ± 2.4cD 4.6 ± 1.1dE 1.8 ± 0.5cE 0.0 ± 0.0eE 0.0 ± 0.0eE 0.0 ± 0.0dE 0.0 ± 0.0dE
T‐M‐SL3% 82.6 ± 4.0bA 44.9 ± 7.5cB 27.5 ± 4.2cC 7.5 ± 1.4cdD 2.8 ± 1.3dE 1.1 ± 0.4cE 0.0 ± 0.0eE 0.0 ± 0.0eE 0.0 ± 0.0dE 0.0 ± 0.0dE
T‐M‐SL5% 80.2 ± 2.4bA 37.8 ± 2.8cB 23.5 ± 3.3cC 2.4 ± 1.0dD 1.1 ± 0.3dD 0.0 ± 0.0cD 0.0 ± 0.0eD 0.0 ± 0.0eD 0.0 ± 0.0dD 0.0 ± 0.0dD
PM (%) T‐EY 66.1 ± 3.3cdeA 61.8 ± 5.0bA 58.5 ± 5.8aAB 56.7 ± 6.5aAB 52.0 ± 3.0aBC 48.9 ± 4.6aCD 46.7 ± 5.5aCD 43.4 ± 6.3aD 39.4 ± 4.0aE 32.2 ± 2.9aF
T‐SL1% 80.5 ± 2.2abA 68.9 ± 1.1abB 62.7 ± 1.2aB 42.5 ± 4.9bC 31.7 ± 5.9bD 24.8 ± 6.1bcE 11.5 ± 1.3cdF 3.5 ± 1.5cG 1.7 ± 0.3cG 0.0 ± 0.0cG
T‐SL3% 82.0 ± 2.3aA 73.9 ± 2.2aB 60.9 ± 4.6aC 44.8 ± 5.8bD 36.9 ± 5.0bE 27.6 ± 5.0bF 16.6 ± 3.8cG 4.4 ± 1.1cH 2.4 ± 0.5cH 0.0 ± 0.0cH
T‐SL5% 74.3 ± 1.6abcA 65.3 ± 1.8bB 45.5 ± 5.6bC 38.6 ± 5.3bD 19.5 ± 2.8cE 13.4 ± 2.6cF 4.3 ± 0.5deG 1.7 ± 0.3cG 0.0 ± 0.0cG 0.0 ± 0.0cG
T‐M‐EY 70.5 ± 4.8bcdA 67.3 ± 5.0abAB 62.3 ± 4.1aABC 59.5 ± 3.3aBC 55.6 ± 7.5aC 44.2 ± 9.1aD 32.4 ± 4.9bE 22.0 ± 3.3bF 7.7 ± 3.6bG 3.6 ± 1.3bH
T‐M‐SL1% 61.1 ± 6.7deA 25.9 ± 3.0cB 11.1 ± 3.0cC 0.0 ± 0.0cD 0.0 ± 0.0dD 0.0 ± 0.0dD 0.0 ± 0.0eD 0.0 ± 0.0cD 0.0 ± 0.0cD 0.0 ± 0.0cD
T‐M‐SL3% 57.5 ± 2.6eA 18.7 ± 2.5cdB 11.6 ± 1.7cC 0.0 ± 0.0cD 0.0 ± 0.0dD 0.0 ± 0.0dD 0.0 ± 0.0eD 0.0 ± 0.0cD 0.0 ± 0.0cD 0.0 ± 0.0cD
T‐M‐SL5% 58.2 ± 6.6eA 12.6 ± 2.3 dB 5.4 ± 2.0cC 0.0 ± 0.0cC 0.0 ± 0.0dC 0.0 ± 0.0dC 0.0 ± 0.0eC 0.0 ± 0.0cC 0.0 ± 0.0cC 0.0 ± 0.0cC

Values are mean ± SD for four replicates, each being a pool of five ejaculates.

Lowercase superscript letters (a, b, c, d or e) in the same column indicates significant difference among extenders (P < 0.05) and uppercase superscript letters (A, B, C, D, E, F, G or H) in the same row indicates significant difference within extenders with different storage time (P < 0.05).