Skip to main content
. 2019 Mar 8;5(3):345–360. doi: 10.1002/vms3.158

Table 4.

Effects of egg yolk (EY) 20% and soybean lecithin (SL) at different concentrations (1%, 3% and 5%) in Tris‐citric‐fructose (T) or Tris‐citric‐fructose‐mineral salts (T‐M) extender on average pathway velocity (VAP), straight line velocity (VSL) and curvilinear velocity (VCL) parameters of chilled canine sperm during a storage period of 10 days at 5°C

Parameters Extenders Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Day 7 Day 8 Day 9 Day 10
VAP (μm s−1) T‐EY 89.6 ± 0.5abA 76.3 ± 5.2aB 71.9 ± 3.5aB 67.6 ± 6.5aB 66.4 ± 7.3aB 64.8 ± 7.6aB 62.9 ± 7.8aB 60.8 ± 7.5aBC 59.1 ± 7.8aC 55.6 ± 8.4aC
T‐SL1% 93.5 ± 0.8aA 76.1 ± 5.0aB 67.8 ± 7.2aBC 56.5 ± 6.5aC 51.6 ± 4.9bD 47.1 ± 6.4bE 44.9 ± 4.8bE 41.4 ± 8.0bE 35.7 ± 6.9bcF 0.0 ± 0.0cG
T‐SL3% 91.6 ± 1.6abA 76.9 ± 2.9aB 67.6 ± 8.7aBC 56.3 ± 7.0aCD 53.2 ± 7.8bD 46.9 ± 4.7bE 43.3 ± 1.9bE 35.7 ± 1.6bcF 32.7 ± 3.3cF 0.0 ± 0.0cG
T‐SL5% 90.8 ± 4,4abA 78.4 ± 5.8aA 64.1 ± 6.7aB 55.9 ± 5.9aB 51.9 ± 5.7bB 43.6 ± 2.9bC 38.1 ± 7.5bD 27.1 ± 2.3cE 0.0 ± 0.0dF 0.0 ± 0.0cF
T‐M‐EY 92.9 ± 4.0aA 78.2 ± 4.3aAB 71.6 ± 7.0aB 64.9 ± 6.0aBC 63.1 ± 6.0abC 60.5 ± 6.1aC 58.6 ± 5.9aCD 53.3 ± 7.2abD 46.8 ± 7.9bE 43.1 ± 7.8bE
T‐M‐SL1% 82.3 ± 7.0abA 59.7 ± 9.4bB 38.6 ± 9.0bC 0.0 ± 0.0bD 0.0 ± 0.0cD 0.0 ± 0.0cD 0.0 ± 0.0cD 0.0 ± 0.0dD 0.0 ± 0.0dD 0.0 ± 0.0cD
T‐M‐SL3% 79.6 ± 6.3bA 47.2 ± 6.9bB 37.1 ± 6.0bB 0.0 ± 0.0bC 0.0 ± 0.0cC 0.0 ± 0.0cC 0.0 ± 0.0cC 0.0 ± 0.0dC 0.0 ± 0.0dC 0.0 ± 0.0cC
T‐M‐SL5% 79.7 ± 9.8bA 55.0 ± 8.9bB 35.6 ± 5.5bC 0.0 ± 0.0bD 0.0 ± 0.0cD 0.0 ± 0.0cD 0.0 ± 0.0cD 0.0 ± 0.0dD 0.0 ± 0.0dD 0.0 ± 0.0cD
VSL (μm s−1) T‐EY 77.0 ± 2.8abA 62.1 ± 7.2abAB 52.1 ± 4.0abBC 47.4 ± 8.1abBCD 45.0 ± 6.9abCD 44.3 ± 6.9abCDE 42.5 ± 6.4abCDE 40.3 ± 4.4aCDE 39.2 ± 4.0aDE 36.3 ± 3.3aE
T‐SL1% 74.0 ± 2.2abA 59.4 ± 4.4abAB 46.9 ± 5.7bBC 41.4 ± 6.8bC 34.8 ± 3.2bD 33.8 ± 3.4bcD 33.1 ± 2.7bcD 29.7 ± 2.6bD 23.0 ± 3.2bE 0.0 ± 0.0cF
T‐SL3% 72.6 ± 5.8abA 58.1 ± 6.3abAB 47.2 ± 7.1bBC 39.4 ± 4.7bC 37.1 ± 5.9bC 31.2 ± 4.2cD 28.1 ± 2.3cE 24.2 ± 2.0bcE 22.4 ± 2.1bE 0.0 ± 0.0cF
T‐SL5% 72.1 ± 8.5abA 56.4 ± 6.2abB 44.8 ± 6.4bBC 36.6 ± 3.9bC 34.5 ± 2.8bC 29.1 ± 3.9cD 25.9 ± 6.4cE 15.6 ± 2.2cF 0.0 ± 0.0cG 0.0 ± 0.0cG
T‐M‐EY 86.1 ± 4.6aA 69.1 ± 3.7aB 63.2 ± 7.7aB 55.5 ± 8.2aB 53.5 ± 8.0aB 49.2 ± 8.1aC 47.6 ± 7.3aCD 41.3 ± 1.0aD 33.6 ± 4.1aE 31.6 ± 4.0bE
T‐M‐SL1% 71.6 ± 8.4abA 51.1 ± 7.6bB 28.0 ± 4.4cC 0.0 ± 0.0cD 0.0 ± 0.0cD 0.0 ± 0.0dD 0.0 ± 0.0dD 0.0 ± 0.0dD 0.0 ± 0.0cD 0.0 ± 0.0cD
T‐M‐SL3% 71.2 ± 6.2abA 45.6 ± 7.9bcB 29.3 ± 4.2cC 0.0 ± 0.0cD 0.0 ± 0.0cD 0.0 ± 0.0dD 0.0 ± 0.0dD 0.0 ± 0.0dD 0.0 ± 0.0cD 0.0 ± 0.0cD
T‐M‐SL5% 65.8 ± 9.4bA 32.6 ± 8.2cB 25.5 ± 6.4cB 0.0 ± 0.0cC 0.0 ± 0.0cC 0.0 ± 0.0dC 0.0 ± 0.0dC 0.0 ± 0.0dC 0.0 ± 0.0cC 0.0 ± 0.0cC
VCL (μm s−1) T‐EY 148.0 ± 6.5bcA 142.1 ± 7.4bcAB 135.8 ± 8.6aABC 127.2 ± 15.3aABCD 124.8 ± 17.1aABCD 121.8 ± 19.5aBCD 119.3 ± 17.7aBCD 115.7 ± 17.6aBCD 112.7 ± 15.6aCD 108.3 ± 14.4aD
T‐SL1% 181.1 ± 8.8aA 168.4 ± 10.9aAB 144.9 ± 13.8aB 116.1 ± 19.0aC 104.2 ± 19.0aD 98.6 ± 19.0aD 87.1 ± 24.1abE 58.6 ± 27.0bF 46.6 ± 13.9cF 0.0 ± 0.0cG
T‐SL3% 172.3 ± 1.6aA 165.0 ± 7.6abAB 143.6 ± 18.9aBC 118.7 ± 17.6aC 109.4 ± 24.6aD 97.5 ± 15.0aE 88.5 ± 6.3abE 62.2 ± 8.6bF 54.8 ± 73.6cF 0.0 ± 0.0cG
T‐SL5% 164.3 ± 5.3abA 158.7 ± 8.7abAB 134.6 ± 17.5aBC 108.7 ± 25.8aC 97.2 ± 23.8aD 89.4 ± 21.1aD 75.4 ± 29.5bE 42.2 ± 10.2bF 0.0 ± 0.0dG 0.0 ± 0.0cG
T‐M‐EY 133.5 ± 3.2cA 129.3 ± 3.9cdAB 123.1 ± 6.1aABC 117.1 ± 2.8aABC 112.9 ± 3.3aABC 110.1 ± 4.0aABC 106.0 ± 5.9abABCD 101.7 ± 9.6aBCD 90.6 ± 11.0bCD 84.4 ± 12.7bD
T‐M‐SL1% 134.2 ± 8.7cA 121.8 ± 7.5cA 72.7 ± 19.0bB 0.0 ± 0.0bC 0.0 ± 0.0bC 0.0 ± 0.0bC 0.0 ± 0.0cC 0.0 ± 0.0cC 0.0 ± 0.0dC 0.0 ± 0.0cC
T‐M‐SL3% 133.2 ± 7.2cA 114.7 ± 16.8 dB 78.9 ± 14.4bC 0.0 ± 0.0bD 0.0 ± 0.0bD 0.0 ± 0.0bD 0.0 ± 0.0cD 0.0 ± 0.0cD 0.0 ± 0.0dD 0.0 ± 0.0cD
T‐M‐SL5% 131.9 ± 9.3cA 87.9 ± 10.5eB 59.4 ± 9.1bC 0.0 ± 0.0bD 0.0 ± 0.0bD 0.0 ± 0.0bD 0.0 ± 0.0cD 0.0 ± 0.0cD 0.0 ± 0.0dD 0.0 ± 0.0cD

Values are mean ± SD for four replicates, each being a pool of five ejaculates.

Lowercase superscript letters (a, b, c, d or e) in the same column indicates significant difference among extenders (P < 0.05) and uppercase superscript letters (A, B, C, D, E, F or G) in the same row indicates significant difference within extenders with different storage time (P < 0.05).