Table 6.
Percentage of healthy sperm with intact plasma membrane, intact acrosome membrane and high mitochondrial membrane potential in chilled canine sperm diluted in Tris‐citric‐fructose (T) or Tris‐citric‐fructose‐mineral salts (T‐M) extender plus 20% egg yolk (EY) or soybean lecithin (SL) at different concentrations (1%, 3% and 5%) during a storage period of 10 days at 5°C
| Extenders | Day 1 | Day 2 | Day 3 | Day 4 | Day 6 | Day 8 | Day 10 |
|---|---|---|---|---|---|---|---|
| T‐EY | 42.2 ± 7.0aA | 31.9 ± 3.6bB | 27.4 ± 3.6bC | 22.2 ± 5.1bD | 13.6 ± 3.2bE | 11.4 ± 2.9aF | 5.4 ± 1.8aG |
| T‐SL1% | 25.9 ± 4.7bcA | 18.8 ± 1.2cB | 13.7 ± 2.2cC | 6.9 ± 1.5cD | 1.3 ± 0.5cE | 0.1 ± 0.0cE | 0.0 ± 0.0bE |
| T‐SL3% | 17.5 ± 2.5cdA | 8.0 ± 0.6deB | 4.9 ± 1.8dBC | 2.2 ± 0.4cdBC | 1.3 ± 0.5cC | 0.3 ± 0.1cC | 0.0 ± 0.0bC |
| T‐SL5% | 14.8 ± 1.8dA | 5.2 ± 1.7eB | 3.3 ± 2.1dBC | 2.2 ± 1.4cdCD | 0.8 ± 0.2cD | 0.1 ± 0.0cD | 0.0 ± 0.0bD |
| T‐M‐EY | 46.8 ± 7. 9aA | 39.0 ± 4.2aB | 36.2 ± 4.0aB | 29.1 ± 2.3aC | 20.8 ± 2.5aD | 6.9 ± 1.6bE | 4.6 ± 0.8aE |
| T‐M‐SL1% | 30.6 ± 2.0bA | 16.3 ± 0.8cB | 7.8 ± 1.6cdC | 6.8 ± 1.3cdC | 1.7 ± 1.0cD | 0.3 ± 0.1cD | 0.0 ± 0.0bD |
| T‐M‐SL3% | 20.2 ± 1.6bcdA | 13.7 ± 3.4cdB | 6.9 ± 1.5dC | 2.4 ± 0.8cdD | 0.45 ± 0.1cD | 0.0 ± 0.0cD | 0.0 ± 0.0bD |
| T‐M‐SL5% | 20.0 ± 1.7bcdA | 8.6 ± 2.1deB | 2.2 ± 1.0dC | 0.8 ± 0.3dC | 0.1 ± 0.0cC | 0.0 ± 0.0cC | 0.0 ± 0.0bC |
Values are mean ± SD for four replicates, each being a pool of five ejaculates.
Lowercase superscript letters (a, b, c, d or e) in the same column indicates significant difference among extenders (P < 0.05) and uppercase superscript letters (A, B, C, D, E, F or G) in the same row indicates significant difference within extenders with different storage time (P < 0.05).