Fig. 5.

Process of the cell counting of the central endothelium for cornea 6: (a) 2-D en face image of the flattened endothelial layer, (b) the cropped image of (a) within a circle of $500\text{-}\mu \mathrm{m}$-in diameter, (c) the endothelial cell borders found using a custom corner method with the manual corner identifications, (d) the corrected borders in (c), and (e) the cell counting using the “analyze particles…” plugin in Fiji.