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The results of the sulfur oxidation of thioanisole for P450 119 and its mutants at 35 °C and pH 7.5 in presence of 20 mM TBHP.
| Entry | Enzyme | Yield (%) 2/3 [a] | ee (%) 2 [b] | Conf. 2 [c] |
|---|---|---|---|---|
| 1 | Wild‐Type | 59.3/30.3 | 16.9 | R |
| 2 | T213G | 72.0/14.5 | 0.2 | R |
| 3 | T213M | 10.3/– | – | – |
| 4 | T213H | 15.4/– | – | – |
| 5 | F153G | 71.3/22.2 | 22.9 | R |
| 6 | F153A | 53.4/29.5 | 19.9 | R |
| 7 | F153V | 60.6/21.9 | 17.5 | R |
| 8 | F153G/T213G | 75.1/11.3 | 6.8 | R |
| 9 | No. 3–39 | 76.2/6.9 | 14.8 | R |
| 10 | K199E | 70.2/18.5 | 12.3 | R |
