Table 4.
The results of the sulfur oxidation of thioanisole for P450 119 and its mutants at pH 6.5 with octanoic acid and pH 8.5 without octanoic acid.
| Entry | Enzyme | pH 6.5[b] | pH 8.5 |
|---|---|---|---|
| Yield of 2/3 (%)[a] | Yield of 2/3 (%)[a] | ||
| 1 | Wild‐Type | 79.1/15.4 | 65.6/38.2 |
| 2 | T213G | 74.4/13.0 | 55.4/35.8 |
| 3 | T213M | 10.9/– | 39.2/0.36 |
| 4 | T213H | 14.1/– | 36.9/0.4 |
| 5 | F153G | 80.2[c]/13.5 | 63.3/23.8 |
| 6 | F153A | 72.8/10.1 | 59.8/27.3 |
| 7 | F153V | 87.7/10.3 | 58.7/32.9 |
| 8 | F153G/T213G | 90.1/9.4 | 79.2/9.3 |
| 9 | No. 3–39 | 88.9/8.2 | 82.8/6.5 |
| 10 | K199E | 80.4/14.6 | 79.3/16.0 |
[a] yield of 2 and 3 determined by GC‐MS, [b] concentration of octanoic acid was 20 mM, [c] enantioselectivity 30 % ee determined by HPLC and R‐configuration determined by comparing the HPLC data with the literature 21.