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Immunoblot of CM sample extracts under reducing conditions referred to pasteurized CM (Lane 1), baked CM at 180 °C for 10 min (Lane 2), blank muffin (Lane 3), and CM incurred muffin (Lane 4). M: MW reference standard The immunoblot was carried out on a pool of sera of young patients (mean age ± standard deviation: 8.5 ± 4.3 years) with a clinical allergy to CM proteins.
