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. 2019 Jul 20;11(7):1665. doi: 10.3390/nu11071665

Figure 4.

Figure 4

(A) Fluorescent LDL-uptake assay after treatment of HepG2 with T9. HepG2 cells were transfected and subsequently incubated with T9 for 24 h. Then, the specific fluorescent LDL uptake was measured by the Synergy H1 fluorescent plate reader. Data points represent averages ± S.D. of three independent experiments in triplicate; (*) p < 0.05. (B) Cell surface LDLR levels in HepG2 cells. An ICW is a colorimetric cell-based assay where cell membranes are not permeabilized, useful to detect the up-regulation of specific LDLR protein target on the cell surface. Bars represent averages ± S.D. of three independent experiments. (*) p < 0.05 and (****) p < 0.00001.