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. 2019 Jul 16;11(7):1612. doi: 10.3390/nu11071612

Figure 4.

Figure 4

Expression of type IV collagen, SIRT1, and HSP70 in the liver. Control; control group (n = 5), HFCS; HFCS group (n = 5). The numbers 1–5 indicate each rat in the control or HFCS group. Tissue lysates (15 μg of protein/lane) were loaded on 4–15% gradient polyacrylamide gels. Proteins on polyvinylidene difluoride (PVDF) membranes were probed with anti-type IV collagen, anti-SIRT1, anti-HSP70, and anti-β-actin antibodies. (a) Type IV collagen and β-actin bands were analyzed with Western blotting (WB). (b) SIRT1 and β-actin bands were analyzed with WB. (c) HSP70 and β-actin bands were analyzed with WB. (d) The expression of type IV collagen in the control and HFCS groups was normalized with β-actin. (e) The expression of SIRT1 in the control and HFCS groups was normalized with β-actin. (f) The expression of HSP70 in the control and HFCS groups was normalized with β-actin. (a–c) β-actin was used as the loading control. (d–f) Data are shown as means ± S.E. (n = 5). p-Values were based on the Mann-Whitney U-test.