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. 2019 Jul 16;11(7):1612. doi: 10.3390/nu11071612

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Calculation of intracellular TAGE levels in Sprague Dawley rat primary hepatocytes. Control; control medium (n = 4), High fructose; high fructose medium (n = 4). Cell lysates (4.0 μg of protein/lane) were loaded on PVDF membranes, and proteins were probed with the anti-TAGE antibody or neutralized anti-TAGE antibody. The amount of TAGE was calculated based on a standard curve for TAGE-BSA. Data are shown as means ± S.D. (n = 4). p-Values were based on the Mann-Whitney U-test. ** p < 0.01 vs. control.