Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 5;9:11324. doi: 10.1038/s41598-019-47815-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Effect of rFXIII-A calcium binding site mutants on its specific activity and substrate specificity. Panels a, b and c represent comparative bar graphs for Specific activity of intracellular lysates (wild type and variant) based on Photometric assay, interpolated FXIII-A activity based on Pentylamine incorporation assay and α-2 antiplasmin incorporation assay, respectively. In the event a variant is significantly different than the wild type, it is marked with a star (*) sign on top of the corresponding bar. Statistical significance is set at p < 0.05. Experiments were performed in duplicates, thrice (N = 6). Panel d shows the putative fibrinogen, α-2 antiplasmin and BAPA binding site residues on zymogenic FXIII-A monomeric and activated monomeric FXIII-A* structures along with two intermediate transition state structures. The structure backbone is depicted in grey colored ribbon format. The putative binding site region backbones are colored cyan for fibrinogen, yellow for BAPA and magenta for α-2 antiplasmin. The three calcium binding sites are also depicted on all four structures as stick models colored red (Cab1), blue (Cab2) and green (Cab3) respectively.