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. 2019 Aug 5;9:11350. doi: 10.1038/s41598-019-47363-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of ZEB1 and ZEB1-AS1 gene expression in TCGA melanoma samples. (a,b) High positive correlation between ZEB1 and ZEB1-AS1 expression in primary melanoma and metastatic melanoma samples, respectively. Analysis from Pearson’s statistical test. Grey band represents the confidence interval. (c) Graph showing higher expression of ZEB1-AS1 in metastatic melanoma compared to primary melanoma samples from SKCM TCGA. T-test analysis was used. (d,e) Boxplot showing expression levels of ZEB1-AS1 or ZEB1, respectively, across different mutational groups of melanoma. Statistics based on ANOVA test. P-values statistical significance labels: *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001.