Fig. 5. Nuclear translocation of AIF and EndoG in HBE cells from non-smokers (n = 7) and smokers (n = 14).

Cultures were exposed to the smoke of 8 cigarettes or an equivalent volume of clean air, followed by isolation of mitochondrial and nuclear fractions three hours post exposure treatment. AIF and EndoG in mitochondrial (a) and nuclear (b) fractions were quantified by western blotting and normalized to β-actin. Data are presented as fold change, where the normalized quantity of each protein for each subcellular fraction in smoke exposed cultures is divided by the quantity of the identical protein in the same subcellular fraction in the air exposed cultures originating from the same donor; mean + SEM, *p ≤ 0.05