Fig. 5. miR-425 knockdown induced age-dependent dopaminergic degeneration.

a Chromogenic ISH of miR-425 expression in Mir-425^low mice at 3 months (3 Mo), 6 months (6 Mo), 15 months (15 Mo) and in WT mice. b Immunohistochemistry for TH and cresyl violet-positive cells in Mir-425^low mice at 3 months, 6 months, 15 months and in WT mice. c Quantification of miR-425 level, TH-positive neuronal fibers in the striatum and TH-positive neurons in the SNpc. d Fluro-Jade B staining in SNpc of Mir-425^low mice and WT mice at 3 months and 15 months. e Study design of MPTP-treated Mir-425^low mice and WT control mice. f Chromogenic ISH of miR-425 expression of MPTP-treated Mir-425^low mice and WT control mice. g Quantification of miR-425 expression in MPTP-treated Mir-425^low mice and WT control mice. h Quantification of TNFα levels in MPTP-treated Mir-425^low mice and WT control mice using ELISA. All data represent the mean ± SEM. Two-way ANOVA followed by post hoc Dunnett’s test was applied, *P < 0.05 and ***P < 0.001, ns, not significant