Fig. 6. miR-425 knockdown exacerbated MPTP-induced dopaminergic neuron loss and impaired locomotor behaviors.

a Immunohistochemistry for TH in the striatum and SN and cresyl violet-positive cells of MPTP-treated Mir-425^low mice. b Quantification of TH-positive neuronal fibers in the striatum and TH-positive neurons and cresyl violet-positive cells in the SNpc. c Immunohistochemistry and quantification of dopamine transporter (DAT) in the SNpc. d Immunoblotting of RIPK1, RIPK3, MLKL, and pMLKL expression in the SNpc of MPTP-treated Mir-425^low and WT mice. e Quantification of RIPK1, RIPK3, MLKL, and pMLKL expression in the midbrain of MPTP-treated Mir-425^low and WT mice. f Representative tracks of mice in the open field chamber over 5 min. g Locomotor behavior in the open field in MPTP-treated Mir-425^low and WT mice, whole-area distance and central-area distance were measured. i Time spent on the rotarod for MPTP-treated Mir-425^low and WT mice was measured. All data represent the mean ± SEM. Student’s t test, *P < 0.05, **P < 0.01, ***P < 0.001, and ns, not significant