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. 2019 Aug 5;9:11321. doi: 10.1038/s41598-019-47475-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Calibration process. An iterative calibration process was used to find the appropriate design dimensions to create ports (a,b), channels (c,d) and barriers between cell culture chambers (e,f) in PDMS using 3D-printed molds. (b,d,f) Representative images of PDMS structures formed after adjustment of the mold CAD files based on the obtained correction factors; the measured feature lengths are indicated in the images and the intended feature lengths shown above each panel (all units = μm). It was not possible to consistently create barriers that were narrower that 200 μm.