Figure 1.

Immunohistochemistry for CD31, adipocyte size and capillary density analysis in human SAT and VAT. Representative photomicrographs of SAT and VAT sections from the 4 groups of patients studied: lean control subjects, normoglycemic (ob N), prediabetic (ob preDM) and diabetic (ob T2DM) obese patients stained with anti-CD31 antibody (A). Measurements of adipocyte area (µm²) and quantification of capillary density (number of capillaries per mm²) in the SAT (white boxes, B,D) and VAT (grey boxes, C,E) of 10 lean, 5 ob N, 5 ob preDM and 5 ob T2DM patients. The results are displayed as box plot graphs: the box represents the lower and upper quartiles, the line in the box represents the median, the whiskers show the lowest and highest values and the outliers indicating the median value of measurements for one field are represented by black circles. Data was analyzed using One Way ANOVA (Fisher LSD method) in (B) and Kruskal-Wallis test (Dunn’s method) in (C–E), *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.