Fig. 1. Mouse NLRP1 allele sensitivity to autoproteolysis and LT cleavage.

a Domain organization of rodent NLRP1 proteins. The LF cleavage and FIIND autoproteolysis sites are indicated (residues correspond to the NLRP1B1 allele). The cartoon is not drawn to scale. b HEK 293T cells were transiently transfected with plasmids encoding the indicated C-terminally FLAG-tagged mouse NLRP1 alleles (2 µg, 48 h) before protein expression was evaluated by immunoblotting. FL full-length. c HEK 293T cells were transiently transfected with the indicated N-terminally V5-GFP-tagged mouse NLRP1 alleles (2 µg, 48 h). Cell lysates were then treated with LF (1 µg/mL) for the indicated times before reactions were quenched with 2× loading dye and LF cleavage evaluated by immunoblotting. Asterisks indicate background bands