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. 2019 Aug 5;2:288. doi: 10.1038/s42003-019-0530-3

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© The Author(s) 2019, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — The COXi treatment effect is dependent on route of infection, not a specific COX1/2 inhibitor. a Female C3HeB/FeJ or CB6F1 mice of 6 to 8-weeks of age were infected by intravenous (i.v.) injection of 5 × 10⁴ Mtb H37Rv or Erdman respectively. After 3 weeks, the animals were put on a 1-week course of 80 mg/day oral ibuprofen before enumerating Mtb bacilli in the lungs (n = 13). b The frequency of neutrophils in the lungs of C3HeB/FeJ mice (n = 4) was determined by flow cytometry gating on CD11b⁺/Ly-6G⁺ cells 1 week after treatment. c Soluble IL-6 was measured along with other pro-inflammatory cytokines (Supplementary Fig. 3) by multiplex analysis of lung homogenates of C3HeB/FeJ mice after 1 week of treatment (n = 4). For this analysis, q values (adjusted p values) were calculated with an ANOVA followed by the Benjamini-Hochberg method to control the false discovery rate during multiple comparisons. d 6 to 8-week-old female CB6F1 mice were infected with a low dose of ~25–50 CFU Mtb Erdman by the aerosol (AE) route and put on a course of 80 mg/day oral ibuprofen at week 4 of the infection (as in Fig. 1). After 8 weeks of treatment (week 12 of the infection), Mtb bacilli were enumerated in both lungs and spleen by plating organ homogenates (n = 8). Data is shown from one out of two independent experimentswith n = 8 and n = 6, respectively. e Two weeks into the treatment (week 6 of the infection) the frequency of neutrophils in the lungs was determined as before (data from one representative experiment, n = 8). f 6 to 8-week-old female CB6F1 mice were infected with a high-dose of ~150–300 CFU Mtb Erdman by the aerosol (AE) route and put on a course of 80 mg/day oral ibuprofen at week 3 of the infection. Mice were monitored individually for 1 week and euthanized when they reached pre-defined humane endpoints. The p value was calculated using a chi-square test. Bars represent mean ± SEM and p values were calculated with an unpaired two-tailed t-test comparing treated and non-treated animals (unless otherwise indicated)