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. 2019 Aug 5;9:11335. doi: 10.1038/s41598-019-47799-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of cellular uptake by BMDCs. BMDCs were treated with Cy5-siRNA loaded in the YSK12-MEND, RNAiMAX or GeneSilencer at siRNA doses of 10 and 20 nM. After incubation for 2 h (YSK12-MEND and RNAiMAX) or for 4 h (GeneSilencer), the cells were collected and analyzed by flow cytometry. A) Typical histogram. B) Average data of relative FI. Data are the mean ± SEM (n = 3, *P < 0.05, **P < 0.01).