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. 2019 Aug 5;9:11294. doi: 10.1038/s41598-019-47746-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Impact of several natural flavonoids on the in vitro affinity of HsrA for its target promoter. (A) EMSAs showing the ability of HsrA to specifically bind in vitro the promoter region of porGDAB operon. DNA fragments were mixed with increasing concentrations of recombinant HsrA protein (indicated in μM) and separated on a 6% PAGE. A 150-bp DNA fragment of Anabaena gene pkn22 was included as non-specific competitor DNA in all assays. (B) DNA fragments were mixed with 6 μM of recombinant HsrA protein in the presence of 2, 1, 0.5 and 0.1 mM of natural flavonoids.