Fig. 4.

Bayesian phylogenetic reconstruction of 13.1 kb of genome-wide nuclear data (RAD tags) for non-hybrid water frogs from Ticino (in bold) and reference samples. All south-alpine frogs belong to the P. lessonae clade, expect for the five P. ridibundus females collected at STA, which branch to the corresponding P. ridibundus clade. The intron-based phylogeny of Dubey & Dufresnes [29] is provided for comparison (~ 1.6 kb from two markers, including 1.4 kb from SAI-1); “P. n. t. 1”: hemiclone sequenced in the Italian hybridogens P. kl. hispanicus; “P. n. t. 2”: putative endemic lineage sequenced in south-alpine P. lessonae and P. kl. esculentus. Our genomic data clearly rule out the existence of the latter. Country codes used for the reference samples as follows. AL: Albania; FR: France; GR: Greece; IT: Italy; PL: Poland; SRB: Serbia; TR: Turkey