Fig. 7.

PLCε depletion inhibits EMT via PPARβ-mediated Twist1 downregulation. a, b Treatment of sh-PLCε-infected PC3 cells with 1 nM GW501516 and 1 μM GSK3787, cell lysates were collected for western blotting and protein quantification analyses. c, d Protein lysates from sh-PLCε-infected PC3 cells treated with GW501516 and GSK3787 were collected for western blotting and protein quantification analyses. e E-cadherin and N-cadherin transcriptional activity determined by luciferase assay in PC3 cells. f, g Transwell assays were employed to test the cell migratory capacity of PCa cell lines. Magnification × 200. Bars = 500 μm. Data were represented as mean ± SD. of three individual experiments. *p < 0.05, **p < 0.01, and *** p < 0.001 vs. Controls