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. 2019 Aug 5;14:189. doi: 10.1186/s13023-019-1166-6

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Intimal fibrosis thickening instead of proliferation of endothelial cells, inflammatory thrombus and NETosis formation inside preserved vessels and organised thrombus in a TAO patient who received no treatment for many years. a PAS staining. X10 objective lense. Perivascular infiltration of inflammatory cells and the preserved vascular structure of a small artery with early-stage thrombus formation. b PAS staining. X40 objective lense Diffuse intimal thiceking with infiltration of inflamamtory cells in the intima layer. c H&E staining. X40 objective lense. Infiltration of inflammatory cells in all layers of the vessel. d PAS staining. X40 objective lense Organized thrombus with recanalization. The arrow shows basal membrane of intima. e IHC for CD31. X40 objective lense. According to the IHC, the intima thickening was not due to the proliferation of endothelial cells. f IHC for Ki67. X40 objective lens. Negative results support that intima thickening was not due to any active cell proliferation