Table 1.

Primer sequences and positions of single-tube multiplex PCR amplification of PAH gene fragments

Primer name	Sequence (5′-3′)	Detection site	Length (bp)
PAHF1	GCTAGCCTGCCTGCTCT	R1 11X	145
PAHR1	GAAGACAGTGTGGAGTTACTT
PAHF2	TGCCCTGCTTGAGACACC	R176X, Ex6–96A > G	191
PAHR2	CATGGAAGCCACAGTAC TT
PAHF3	CTAGCGTCAAAGCCTATGT	R241C, R243Q, R252Q	338
PAHR3	ATGAACCCAAACCTCATTC
PAHF4	TATGGGATGCAGCAGGG	Y356X, V399 V	274
PAHR4	ACCACCCACAGATGAGTGG
PAHF5	TTCTCCAAATGGTGCCCTT	R413P	232
PAHR5	GCGATGGTAGGGAAAGACAG