Table 3.

Hybridization components used in molecular hybridization analysis

Hybrid fluid	Ingredient
Liquid A	2x SSC, 0.1% SDS
Liquid B	0.5 x SSC, 0.1% SDS
Liquid C	0.1 mol L Sodium citrate
Incubating liquid	0.25 U/mL Streptomycin –POD, 2x SSC, 0.1% SDS
Color-substrate solution	0.0015% hydrogen peroxide, 0.1 mg/mL TMB, 0.1 mol/L Sodium citrate

Note: Streptavidin-POD was purchased from Thermo, USA