Fig. 2.

Silencing of BMP2 represses cell proliferation, invasion, and migration in HCC. a Cell proliferation ability (measured in OD at 570 nm) in response to over-expression of BMP2 or silencing BMP2 measured by MTT assay. b Cell invasion and migration in response to over-expression of BMP2 or silencing BMP2 measured by Transwell assay (× 400). c Western blot analysis of MMP-2, MMP-9, Vimentin, and E-cadherin proteins in response to over-expression of BMP2 or silencing BMP2. Data were expressed as mean ± standard deviation, and data between two groups were compared by Student’s t test. *p < 0.05. BMP2, bone morphogenetic proteins 2; HCC, hepatocellular carcinoma; OD, optical density; NC, negative control; MTT, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H_tetrazolium bromide