Fig. 5.

BMP2 regulates the activation of the MARK/p38 signaling pathway in endothelial cells of HCC. a Bands of total p38, ERK1/2, JNK, and Akt proteins and phosphorylated proteins in ECV304 cells measured by Western blot analysis after over-expression or silencing of BMP2. b Expression of p38, ERK1/2, JNK, and Akt proteins and their phosphorylation extents in ECV304 cells. Relative protein expression values were expressed as mean ± standard deviation, and data between two groups were compared by Student’s t test. *p < 0.05. HCC, hepatocellular carcinoma; BMP2, bone morphogenetic proteins 2; NC, negative control; MAPK, mitogen-activated protein kinase