Fig 5.

Proteomic analysis of EMSY knockdown in a skin organotypic model. A, Volcano plot showing mean fold change in 4 biological replicate samples comparing the nontargeting (NT) control siRNA–treated model with EMSY siRNA knockdown. t Test results are color coded red (P < .05), orange (fold change ≤ 2.5 or ≥ 0.5), or green (P < .05 and fold change ≥ 2.5 or ≤ 0.5). B, Ingenuity Pathway Analysis (Qiagen) of proteins consistently upregulated or downregulated (0.5 ≥ fold change ≥ 2.5) in 3 or more of 4 biological replicates, showing proteins defective in monogenic skin diseases with similarities to AD, including ichthyoses,^{42, 43, 44, 45} hyperkeratosis,⁴⁶ or skin fragility^{47, 48}(red asterisks) and an enhancement of pathways predicted to inhibit the development of dermatitis, hyperkeratosis, and hair disorders (blue cogwheels).