Table 4.
Bacterial counts (CFU/g) of mice skin section at 4, 9, and 14 days after treatment.
| Day | MRSA | Free Van+MRSA | LV+MRSA | Free Lys+MRSA | LysL+MRSA | LysLV+MRSA | Free Lys+Van+MRSA |
|---|---|---|---|---|---|---|---|
| 0 4 9 14 |
3×107 Fatal Fatal Fatal |
5.5×107 5.7×107 ±4.4×107 4.3×105 ±2.8×105 295±22 |
5.5×107 1.8×107 ±2.7×107 3.2×104 ±2.8×104 205±19 |
5.4×107 6.4×105 ±1.8×105 2.5×103 ±50 101±11 |
5.4×107 4.2×106 ±2.7×106 104 ±8.8×103 165±11 |
5.4×107 3.7×105 ±1.1×105 2×102±102 12±3 |
5.4×107 5×105 ±2.1×105 9×102±1.3×102 61±8 |
Notes: A skin region of about 7 mm was removed from the back of the mice, and the wound was infected with 10 μL of broth mix containing 107 CFU of MRSA alone, or together with free vancomycin, free lysostaphin, LV, LysL, free Lys/Van, and LysLV. All mice treated with various formulations were treated at the end of the 14nth day in comparison with the 1st day (p<0.05). The most effective results belonged to the mice treated with LysLV and it significantly reduced the number of bacteria in the surgical site in compression with other formulations at the end of the 9th and 14th days (p<0.05).
Abbreviations: LV, liposomal vancomycin; LysLV, lysostaphin-conjugated liposomal vancomycin; LysL, lysostaphin-conjugated liposomes without vancomycin; Van, vancomycin; Lys, lysostaphin.