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. 2019 Jun 21;151(8):1007–1016. doi: 10.1085/jgp.201812210

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Figure 1. — The bifunctional spin label (BSL). (A) Chemical structure of BSL reacted with two cysteine residues. (B) BSL bound stereospecifically to an α-helix at positions i and i + 4. (C) Angles that define the orientation of the nitroxide (defined by axes x_N, y_N, and z_N) relative to the applied magnetic field B; these angles directly determine the high-resolution orientation dependence of the EPR spectrum. Coordinates of BSL are from Binder et al., 2018 (Preprint).