Figure 1.

Generation of Spry1^Y53A knockin mice. (A) Targeting strategy used, including primers used for PCR screening and genotyping, and Southern blot probes (black horizontal bars). (B) A first screening was performed by using PCR using primers 396F and 409R (top panel). Tail DNA from Spry1^−/− mice served as positive control. PCR-positive clones (asterisk) were confirmed by Southern blot (middle panel), and Spry^Y53ANeo/+ mice were genotyped using primers 429F, 430R, and 431R (lower panel). (C and D) Reduced weight of male and female knockin mice at weaning. P values were calculated by the Mann–Whitney test. (E) Allelic frequencies at weaning (3–4 weeks). S, SpeI; X, XbaI; E, EcoRI; B, BglII; Het, heterozygous; KI, knockin; KO, knockout; ORF, open reading frame; WT, wild type.