Characterization of kidneys from newborn Spry1 knockin and knockout mice. (A) Wilms’ Tumor (WT-1) staining of the ectopic nephrogenic zones. Note WT-1–positive developing and mature nephrons, but not cystic cavities. (B) DBA, LTA, and uroplakin (Up) staining of newborn kidneys from the indicated genotypes. Cysts are DBA-positive, revealing their collecting duct origin. Scale bars, 40 µm for 20×, 200 µm for 4×, and 20 µm for 40× pictures. DAPI, 4′,6-diamidino-2-phenylindole.