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. 2019 Jul 5;30(8):1413–1424. doi: 10.1681/ASN.2018080862

Figure 2.

Figure 2.

Erythrocyte ADORA2B underlies increased 2,3-biphosphoglycerate (2,3-BPG) production and oxygen delivery in the angiotensin II (Ang II)–infused mouse model of CKD. (A) Adenosine levels in plasma of mice at day 14 with Ang II or PBS infusion. n=5–9 mice for each group. (B) Adenosine levels in kidneys of mice at day 14 with Ang II or PBS infusion. n=4–8 mice for each group. *P<0.05. (C) Level of 2,3-BPG in red blood cells collected from the mice at day 14 with Ang II or PBS infusion. n=4–17 mice for each group. *P<0.05. (D) The partial pressure of oxygen to allow 50% of hemoglobin binding to oxygen (P50) value of hemoglobin from mice at days 0 and 14 with Ang II or PBS infusion. n=3–6 mice for each group. *P<0.05. (E) Increased renal hypoxia was observed in ADORA2Bf/f/EpoR-Cre+ mice compared with EpoR-Cre+ mice after 14 days of Ang II treatment by Hypoxyprobe. Red indicates Hypoxyprobe and blue indicates 4′,6-diamidino-2-phenylindole. n=3 mice for each group. Scale bar, 200 µm. *P<0.05. (F) Ratio of microalbumin to creatinine in 24-hour urine collected on days 0 and 14. n=3–9 mice for each group. *P<0.05. (G) Systolic BP was measured at various intervals by the tail cuff method until mice were euthanized at 14 days. n=6–9 mice for each group.