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. 2019 Jul 3;218(8):2659–2676. doi: 10.1083/jcb.201811147

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© 2019 Lee et al.

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Figure 5. — Drg1 knockdown has a mild effect on Dvl localization to the basal body region in MCCs. (A) Drg1 colocalizes with the Dvl2 C terminus in MCCs. The mRNAs of the Dvl2 C-terminal fragment tagged with mCherry and Drg1-GFP were coinjected into both marginal ventral blastomeres of four-cell-stage embryos and fixed at stage 25. Images are generated by maximum intensity projection of serial z-stack confocal images. Scale bar, 10 µm. (B) The C-terminus of Dvl2 associates with Drg1. The indicated HA-tagged Dvl2 mutants were coexpressed with Drg1-Flag, and co-IP was performed. (C) Drg1 knockdown diminishes Dvl2 C-terminal localization to the basal body area. mRNAs of Dvl2-c-term-GFP and centrin-RFP (basal body marker) were coinjected with the indicated MOs and mRNAs. The expression level of Dvl2-c-term-GFP was tested by Western blot. Embryos were fixed at stage 25–27. Serial z-stack images were generated. A maximum intensity projection image is shown. Scale bars, 5 µm.