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. 2019 Jun 21;218(8):2583–2599. doi: 10.1083/jcb.201902061

Figure 2.

Figure 2.

Endogenous Spastin is required for LD–peroxisome contact formation. (A) Association between LDs and peroxisomes in control HeLa cells or HeLa cells treated with cumyl-OOH or As3+ for 30 min. Representative confocal MIP images are shown. (B) Fraction of LD overlapping peroxisome as described in A. Means ± SEM are shown (31–40 cells from three to four independent experiments). (C) Fraction of LD overlapping peroxisome in siCtrl and siSpastin-transfected HeLa cells in control condition or under oxidative stress as described in A. Means ± SEM are shown (21–28 cells from three independent experiments). (D) Relative duration of LD contacting peroxisome (as described in Fig. 1, I and J) in siControl and siSpastin-transfected HeLa cells. Median ± interquartile ranges are shown (64–65 LDs from three independent experiments). **, P < 0.01; ***, P < 0.001.