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. 2019 Jun 21;218(8):2583–2599. doi: 10.1083/jcb.201902061

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© 2019 Chang et al.

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Figure 7. — M1 Spastin controls LD-to-peroxisome FA trafficking. (A) Schematic diagram representing NBD-C12 pulse-chase assay in cells transfected with siRNAs and mCherry-SKL. (B) Distribution of NBD-C12 in BODIPY-665/676–labeled LDs and mCherry-SKL–labeled peroxisomes in HeLa cells transfected with siCtrl or siSpastin. Representative confocal MIP images are shown. (C) Relative NBC-C12 intensity in LDs or peroxisomes as described in B. Means ± SEM are shown (26–30 cells from three independent experiments). **, P < 0.01; ***, P < 0.001. (D) Schematic diagram representing transient NBD-C12 pulse-chase assay in cells transfected with mCherry-SKL alone or mCherry-SKL and Halo-M1 Spastin. (E) Redistribution of NBD-C12 to peroxisomes in Halo-M1 Spastin overexpressing HeLa cells following pulse-chase described in D. Line scans of relative intensity profile are shown (right panels). Representative confocal mages are shown. (F) Relative NBC-C12 intensity in peroxisomes as described in D and E. Means ± SEM are shown (12–13 cells from three independent experiments).