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. 2019 Jul 1;218(8):2677–2698. doi: 10.1083/jcb.201812076

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© 2019 Trotter et al.

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Figure 1. — Schematic of the Nrxn1 cKI mice and impaired survival following constitutive truncation of Nrxn1. (A) Diagram of WT, HA-tagged full-length, and HA-tagged truncated Nrxn1α, Nrxn1β, and Nrxn1γ. Top: WT Nrxn1 proteins. Middle: Nrxn1 proteins containing inserted tandem HA-epitopes and loxP sequences in cKI mice. Bottom: Truncated HA-tagged Nrxn1 proteins after Cre recombination (LNS1–6, LNS1-6 domains; E, EGF-like domain; C, cysteine-loop domain). (B) Amino acid sequences of the juxtamembranous region of WT Nrxn1 (top), Nrxn1 with the HA-epitope/loxP site knockin (middle), and Cre recombined truncated Nrxn1 (bottom; SS5 = alternative SS5). (C) Constitutive Nrxn1 truncation impairs postnatal survival as analyzed in newborn (P1) and 21-d old mouse (P21) offspring from heterozygous matings. Statistical significance was assessed by the chi-square test (**, P < 0.01). For further details, see Fig. S1.