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. 2019 Jul 24;2019:3527809. doi: 10.1155/2019/3527809

Figure 3.

Figure 3

PCA reduced p66shc-mediated ROS formation in ethanol-exposed AML-12 cells. AML-12 cells were transfected with p66shc-specific siRNA or control siRNA for 36 h, 10 μM PCA for 6 h, and/or 100 mM ethanol for 24 h. (a, b) Protein levels of p66shc and MnSOD (n = 3); (c) immunofluorescence staining for the p66shc antibody in AML-12 cells for proliferation analysis. Scale bar = 100 μm. (d) Hepatic CAT levels (n = 10), (e) hepatic GSH levels (n = 10), (f) hepatic MDA levels (n = 10), and (g) hepatic H2O2 levels (n = 10). (h) DCFH-DA staining. Scale bar = 100 μm. ∗∗ P < 0.01 and ∗ P < 0.05 compared with the control group; ## P < 0.01 and # P < 0.05 compared with the ethanol group.