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. 2019 Jul 24;2019:3527809. doi: 10.1155/2019/3527809

Figure 4.

Figure 4

miR-219a-5p decreased the p66shc expression in ALD. (a) qRT-PCR analysis of the miRNA expression in different groups of rats (n = 6). ∗∗ P < 0.01 and ∗ P < 0.05 compared with ND; ## P < 0.01 and # P < 0.05 compared with ALD. (b) qRT-PCR analysis of the miR-219a-5p expression in ethanol-exposed AML-12 cells (n = 6). ∗∗ P < 0.01 and ∗ P < 0.05 compared with the control group; ## P < 0.01 and # P < 0.05 compared with the ethanol group. (c) The miR-219a-5p target sequence in the p66shc 3′-UTR is conserved across various species. (d–g) AML-12 cells were transfected with ago-219a or ant-219a to upregulate or inhibit miR-219a-5p. Ago-NC or antago-NC was used as a negative control. (d, e) qRT-PCR analysis of miR-219a-5p expression (n = 6). (f, g) p66shc protein levels (n = 3). ∗∗ P < 0.01 and ∗ P < 0.05 compared with ago-NC or antago-NC.