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. 2019 Jul 24;2019:3527809. doi: 10.1155/2019/3527809

Figure 5.

Figure 5

miR-219a-5p attenuated p66shc-mediated ROS formation in vitro. (a–c) AML-12 cells were cotransfected with ago-219a, si-p66shc, or the corresponding negative control, as indicated. (a) qRT-PCR analysis of the miR-219a-5p expression (n = 6). (b, c) Protein levels of p66shc and MnSOD (n = 3). (d–f) AML-12 cells were cotransfected with ant-219a, si-p66shc, or the corresponding negative control, as indicated. (d) qRT-PCR analysis of the miR-219a-5p expression (n = 6). (e, f) Protein levels of p66shc and MnSOD (n = 3). ∗∗ P < 0.01 and ∗ P < 0.05 compared with the control group; ## P < 0.01 and # P < 0.05 compared with the ethanol group; && P < 0.01 and & P < 0.05 compared with cells transfected with ant-219 with ethanol.