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. 2019 Jul 24;2019:3527809. doi: 10.1155/2019/3527809

Figure 6.

Figure 6

PCA inhibited the p66shc expression via stimulation of miR-219a-5p. (a) Luciferase assay of AML-12 cotransfected with reporter constructs containing p66shc 3′-UTR-wt or 3′-UTR-mut and with ant-219a or antago-NC, as indicated (n = 3). (b) Schematic of the wild-type p66shc 3′-UTR (3′-UTR-wt) and mutated p66shc 3′-UTR (3′-UTR-mut) luciferase constructs. BS: binding site. (c, d) AML-12 cells were transfected with either ant-219a or antago-NC for 24 h and 10 μM protocatechuic acid treatment for 6 h. (c) qRT-PCR analysis of the miR-219a-5p expression (n = 6). (d) Protein levels of p66shc (n = 3). ∗∗ P < 0.01 and ∗ P < 0.05 compared with the antago-NC group; ## P < 0.01 and # P < 0.05 compared with the ant-219a group.