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. 2019 Jul 24;2019:3527809. doi: 10.1155/2019/3527809

Figure 7.

Figure 7

PCA exerted an antioxidant effect through the miR-219a-5p/p66shc pathway with ethanol exposure. AML-12 cells were transfected with antogo-miR-219a-5p or antago-NC for 24 h, treated with 10 μM PCA for 6 h and/or 100 mM ethanol for 24 h. (a) qRT-PCR analysis of miR-219a-5p (n = 6). (b, c) Protein levels of p66shc and MnSOD (n = 3). (d) Hepatic GSH levels (n = 10), (e) hepatic MDA levels (n = 10), (f) hepatic CAT levels (n = 10), and (g) hepatic H2O2 levels (n = 10). (h) DCFH-DA staining. Scale bar = 100 μm. ∗∗ P < 0.01 and ∗ P < 0.05 compared with the control group; ## P < 0.01 and # P < 0.05 compared with the ethanol group; && P < 0.01 and & P < 0.05 compared with cells transfected with ago-NC with ethanol.