Figure 5.

Astragaloside IV could reduce the apoptosis and the excessive autophagy in iron-induced Lo2 cells at the protein level. The expression levels of Bax, Bcl-2, p62 and LC3II / I were determined by real-time PCR (a) and western blot (b). (c) The expression levels of hepcidin were detected by western blot. The control group was given 1 ‰ DMSO, and the model group was given Fe alone. The treatment group was given different concentrations of Astragaloside IV followed with the 60μM Fe. [#, p<0.05, ##, p<0.01, VS Control; ∗∗, p<0.01, ∗∗∗, p<0.001, VS 60Fe].