Table 1.
Primers used for the amplification of transcritps derived from the class II bacteriocin precursor genes of L. plantarum, L. casei, and L. salivarius.
| Gene | Bacteriocin | Primers | |
|---|---|---|---|
| plnE | Plantaricin E, L. plantarum | Fw: caatattccaggttgccgca | Rv: gaatgcctgcaactgaacca |
| plnF | Plantaricin F, L. plantarum | Fw: atttcaggtggcgttttcca | Rv: aatcctcggacagcgctaat |
| plnJ | Plantaricin J, L. plantarum | Fw: gccagcttcgccatcataaa | Rv: aggatttggatgtagtagatgca |
| plnK | Plantaricin K, L. plantarum | Fw: ttgaaccaccaagcacgg | Rv: ttgaagaattaactgctgacgc |
| A | Class IIb bacteriocin, L. casei | Fw: agttgtcaggggtttcaggt | Rv: ccgccgattatcccaaaagg |
| B | Class IIb bacteriocin, L. casei | Fw: gagccaagcgacgcaataaa | Rv: cgcctgcaacagttgtaaatg |
| Tα | Class IId bacteriocin, L. salivarius | Fw: gcaatcagaggaggaatggc | Rv: ccgatacaagccaatccacc |
| Tβ | Class IId bacteriocin, L. salivarius | Fw: gggaatggcattaattgggga | Rv: ggattaccgaaagctgcacc |
| gyrA | DNA topoisomerase, L. plantarum | Fw: tttaagtcgcaacaccgtgg | Rv: gattcctttggccgtacgac |
| dnaG | DNA primase, L. plantarum | Fw: agttggtagtcggtctggtg | Rv: cgcacctaaggatcagcaac |
| gyrA | DNA topoisomerase, L. paracasei | Fw: cttccacgcatgatgtcctg | Rv: cgccttcatgcacgttgata |
| dnaG | DNA primase, L. paracasei | Fw: cagttcggccaattgatcgt | Rv: cgactcgatccaggaatcca |
| gyrA | DNA topoisomerase, L. salivarius | Fw: gttttgccagcacgttttcc | Rv: tcccattacaattgcgccag |
| dnaG | DNA primase, L. salivarius | Fw: gcacaaagattcaacgtcgc | Rv: cgttctgctttctctgcctt |
gyrA and dnaG were selected as housekeeping transcripts as previously recommended (Rocha et al., 2015).