Figure 2.

Human papillomavirus type 16 (HPV16)-E6 antibody median fluorescence intensity (MFI) value in serial samples from OPSCC white cases over the time period leading up to the cancer diagnosis (time 0), for cases who were HPV16-E6 seropositive throughout the entire follow-up period (A), cases who seroconverted during follow-up (B), and cases who were seronegative throughout follow-up (C).The dashed line represents the assay cut-off for HPV16-E6 seropositivity, MFI > 1000. In (B), the case who seroconverted furthest from cancer diagnosis is highlighted in green and the case with multiple measurements who stably seroconverted closest to diagnosis is highlighted in blue. One case fluctuated around the assay threshold for positivity and is HPV16-E6 seropositive in one of their six available serial samples.