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. 2019 Jun 13;216(8):1904–1924. doi: 10.1084/jem.20181657

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© 2019 Bersellini Farinotti et al.

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Figure 4. — FcγRs are expressed in mouse DRG neurons. (A) Microarray data showed mRNA for Fcgr1–4 in CBA mouse DRG (n = 3). (B) Quantitative PCR showed mRNA for Fcgr1–4 in B10.RIII mouse DRG (n = 10). (C) Publicly available RNA sequencing of C57BL/6 mouse DRGs show the presence for Fcgr1–4 (n = 3). (D) smFISH showed mRNA molecules for Fcgr1, Fcgr2b, and Fcgr3 in BALB/c mouse DRG colocalizing with NeuN. Scatter graph shows number of mRNA molecules in neuronal soma. Scale bars represent 100 µm and 10 µm in close-up images. (E) FcγRI protein expression was detected by Western blotting in DRGs from WT BALB/c and B10.RIII mice, but not from FcRγ-chain^−/− mice. (F) Proteomic analysis identified peptides specific for FcγRIIb in BALB/c mouse DRG. See also Figs. S1 and S4.